An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

LINGO-1 protein interacts with the p75 neurotrophin receptor in intracellular membrane compartments.

Meabon JS, De Laat R, Ieguchi K, Wiley JC, Hudson MP, Bothwell M

Axon outgrowth inhibition in response to trauma is thought to be mediated via the binding of myelin-associated inhibitory factors (e.g. Nogo-66, myelin-associated glycoprotein, oligodendrocyte myelin glycoprotein, and myelin basic protein) to a putative tripartite LINGO-1·p75(NTR)·Nogo-66 receptor (NgR) complex at the cell surface. We found that endogenous LINGO-1 expression in neurons in the cortex and cerebellum is intracellular. Mutation or truncation ... [more]

J. Biol. Chem. Apr. 10, 2015; 290(15);9511-20 [Pubmed: 25666623]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

NGFR

Gene Ontology Biological Process

Gene Ontology Molecular Function

Rab GTPase binding [ISS]protein binding [IPI]receptor activity [TAS]signal transducer activity [TAS]transmembrane signaling receptor activity [TAS]ubiquitin protein ligase binding [IPI]

Gene Ontology Cellular Component

LINGO1