BAIT
PSAP
AI037048, SGP-1
prosaposin
GO Process (6)
GO Function (0)
GO Component (7)
Gene Ontology Biological Process
Gene Ontology Cellular Component
Mus musculus
PREY
CST3
CysC, RP23-250M11.9
cystatin C
GO Process (11)
GO Function (5)
GO Component (15)
Gene Ontology Biological Process
- defense response [ISO]
- extracellular fibril organization [ISO]
- negative regulation of cell death [ISO]
- negative regulation of elastin catabolic process [ISO]
- negative regulation of endopeptidase activity [ISO]
- negative regulation of peptidase activity [ISO]
- negative regulation of proteolysis [ISO]
- positive regulation of DNA replication [ISO]
- positive regulation of cell proliferation [ISO]
- regulation of programmed cell death [ISO]
- response to oxidative stress [ISO]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- axon [ISO]
- basement membrane [ISO]
- cell projection [ISO]
- contractile fiber [ISO]
- cytoplasm [ISO]
- endoplasmic reticulum [ISO]
- extracellular region [ISO]
- extracellular space [ISO]
- extracellular vesicular exosome [ISO]
- lysosome [ISO]
- multivesicular body [ISO]
- neuronal cell body [ISO]
- nuclear membrane [ISO]
- perinuclear region of cytoplasm [ISO]
- vesicle [ISO]
Mus musculus
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Prosaposin facilitates sortilin-independent lysosomal trafficking of progranulin.
Mutations in the progranulin (PGRN) gene have been linked to two distinct neurodegenerative diseases, frontotemporal lobar degeneration (FTLD) and neuronal ceroid lipofuscinosis (NCL). Accumulating evidence suggests a critical role of PGRN in lysosomes. However, how PGRN is trafficked to lysosomes is still not clear. Here we report a novel pathway for lysosomal delivery of PGRN. We found that prosaposin (PSAP) ... [more]
J. Cell Biol. Sep. 14, 2015; 210(6);991-1002 [Pubmed: 26370502]
Throughput
- High Throughput
Additional Notes
- assayed using SILAC IP (stable isotope labeling by amino acids in cell culture-based quantitative immunoprecipitation)
Curated By
- BioGRID