CLTB
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
HAP1
Gene Ontology Biological Process
- anterograde axon cargo transport [IMP, ISO]
- cellular response to growth factor stimulus [IEP]
- cerebellum development [ISO]
- early endosome to late endosome transport [TAS]
- hypothalamus cell differentiation [ISO]
- negative regulation of beta-amyloid formation [IMP]
- neuron projection development [IDA]
- neurotrophin TRK receptor signaling pathway [ISO, ISS]
- positive regulation of epidermal growth factor receptor signaling pathway [IMP]
- positive regulation of inclusion body assembly [IDA]
- positive regulation of inositol 1,4,5-trisphosphate-sensitive calcium-release channel activity [IDA, ISO]
- positive regulation of neurogenesis [ISO]
- positive regulation of neuron projection development [IDA]
- positive regulation of neurotrophin production [IDA, IMP]
- positive regulation of nonmotile primary cilium assembly [ISO, ISS]
- positive regulation of synaptic transmission, GABAergic [IDA, IMP]
- protein localization [ISO]
- regulation of exocytosis [ISO, ISS]
- regulation of organelle transport along microtubule [ISO, ISS]
- retrograde axon cargo transport [IMP]
- vesicle transport along microtubule [ISO]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Huntingtin-associated protein-1 (HAP1) regulates endocytosis and interacts with multiple trafficking-related proteins.
Huntingtin-associated protein 1 (HAP1) was initially identified as a binding partner of huntingtin, mutations in which underlie Huntington's disease. Subcellular localization and protein interaction data indicate that HAP1 may be important in vesicle trafficking, cell signalling and receptor internalization. In this study, a proteomics approach was used for the identification of novel HAP1-interacting partners to attempt to shed light on ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| HAP1 CLTB | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | Low | - | BioGRID | - | |
| HAP1 CLTB | Reconstituted Complex Reconstituted Complex An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator. | Low | - | BioGRID | - |
Curated By
- BioGRID