BAIT

SYT1

P65
synaptotagmin I
GO Process (13)
GO Function (15)
GO Component (13)
Rattus norvegicus
PREY

SET

Ab1-115
SET nuclear proto-oncogene
Rattus norvegicus

Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

Specificity of Ca2+-dependent protein interactions mediated by the C2A domains of synaptotagmins.

Sugita S, Suedhof TC

Synaptotagmins represent a family of neuronal proteins thought to function in membrane traffic. The best characterized synaptotagmin, synaptotagmin I, is essential for fast Ca2+-dependent synaptic vesicle exocytosis, indicating a role in the Ca2+ triggering of membrane fusion. Synaptotagmins contain two C2 domains, the C2A and C2B domains, which bind Ca2+ and may mediate their functions by binding to specific targets. ... [more]

Biochemistry Mar. 21, 2000; 39(11);2940-9 [Pubmed: 10715114]

Throughput

  • Low Throughput

Curated By

  • BioGRID