An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

HIC1 deletion promotes breast cancer progression by activating tumor cell/fibroblast crosstalk.

Wang Y, Weng X, Wang L, Hao M, Li Y, Hou L, Liang Y, Wu T, Yao M, Lin G, Jiang Y, Fu G, Hou Z, Meng X, Lu J, Wang J

Breast cancer (BrCa) is the malignant tumor that most seriously threatens female health; however, the molecular mechanism underlying its progression remains unclear. Here, we found that conditional deletion of hypermethylated in cancer 1 (HIC1) in the mouse mammary gland might contribute to premalignant transformation in the early stage of tumor formation. Moreover, the chemokine (C-X-C motif) ligand 14 (CXCL14) secreted ... [more]

J. Clin. Invest. Dec. 03, 2017; 128(12);5235-5250 [Pubmed: 30204129]

Throughput

High Throughput

Additional Notes

Source of CXCL14 not clear

Curated By

BioGRID

Interaction Summary

KLRC1

Gene Ontology Biological Process

Gene Ontology Molecular Function

transmembrane signaling receptor activity [TAS]

Gene Ontology Cellular Component

CXCL14