Reconstituted Complex

An interaction is detected between purified proteins in vitro.

Publication

The sclerostin-bone protein interactome.

Devarajan-Ketha H, Craig TA, Madden BJ, Robert Bergen H, Kumar R

The secreted glycoprotein, sclerostin alters bone formation. To gain insights into the mechanism of action of sclerostin, we examined the interactions of sclerostin with bone proteins using a sclerostin affinity capture technique. Proteins from decalcified rat bone were captured on a sclerostin-maltose binding protein (MBP) amylose column, or on a MBP amylose column. The columns were extensively washed with low ... [more]

Biochem. Biophys. Res. Commun. Jan. 13, 2012; 417(2);830-5 [Pubmed: 22206666]

Throughput

  • High Throughput

Curated By

  • BioGRID