Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Proteomic analysis of an interactome for long-form AMPA receptor subunits.

Santos SD, Manadas B, Duarte CB, Carvalho AL

Glutamate receptors of the AMPA-type mediate fast excitatory synaptic transmission in the central nervous system and play key roles in synaptic plasticity. The binding of these receptors to a variety of proteins is known to regulate their targeting to the synapse and consequently to modulate synaptic strength, as well as to modify receptor characteristics. In this study, a proteomic screening ... [more]

J. Proteome Res. Apr. 05, 2010; 9(4);1670-82 [Pubmed: 20131911]

Throughput

  • High Throughput

Curated By

  • BioGRID