An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Repurposing Brigatinib for the Treatment of Colorectal Cancer Based on Inhibition of ER-phagy.

Zhang Z, Gao W, Zhou L, Chen Y, Qin S, Zhang L, Liu J, He Y, Lei Y, Chen HN, Han J, Zhou ZG, Nice EC, Li C, Huang C, Wei X

Rationale: The sustained and severe endoplasmic reticulum (ER) stress in cancer cells may contribute to apoptotic cell death, thus representing a potential target for cancer therapy. Brigatinib is an anaplastic lymphoma kinase (ALK) inhibitor approved for the treatment of ALK-positive non-small-cell lung cancer (NSCLC). However, it remains unclear if brigatinib has alternative model of action to exert antitumor effect in ... [more]

Theranostics Aug. 15, 2019; 9(17);4878-4892 [Pubmed: 31410188]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

OSBPL8

Gene Ontology Biological Process

Gene Ontology Molecular Function

cholesterol binding [IDA]

Gene Ontology Cellular Component

USP5