UHRF1
Gene Ontology Biological Process
- cell proliferation [IEP]
- histone monoubiquitination [ISS]
- histone ubiquitination [IDA]
- maintenance of DNA methylation [IMP]
- negative regulation of transcription from RNA polymerase II promoter [IDA]
- positive regulation of DNA topoisomerase (ATP-hydrolyzing) activity [IC]
- positive regulation of cellular protein metabolic process [IDA]
- positive regulation of transcription from RNA polymerase II promoter [IC]
- protein autoubiquitination [IDA]
- protein ubiquitination involved in ubiquitin-dependent protein catabolic process [IDA]
Gene Ontology Molecular Function- core promoter proximal region sequence-specific DNA binding [IDA]
- hemi-methylated DNA-binding [IDA]
- histone binding [IDA]
- identical protein binding [ISS]
- methyl-CpG binding [IDA]
- methylated histone binding [IDA]
- nucleosomal histone binding [ISS]
- protein binding [IPI]
- sequence-specific DNA binding transcription factor activity [TAS]
- ubiquitin-protein transferase activity [IDA, ISS]
- zinc ion binding [IDA]
- core promoter proximal region sequence-specific DNA binding [IDA]
- hemi-methylated DNA-binding [IDA]
- histone binding [IDA]
- identical protein binding [ISS]
- methyl-CpG binding [IDA]
- methylated histone binding [IDA]
- nucleosomal histone binding [ISS]
- protein binding [IPI]
- sequence-specific DNA binding transcription factor activity [TAS]
- ubiquitin-protein transferase activity [IDA, ISS]
- zinc ion binding [IDA]
Gene Ontology Cellular Component
LIG1
Gene Ontology Biological Process
- DNA metabolic process [TAS]
- DNA repair [TAS]
- DNA strand elongation involved in DNA replication [TAS]
- V(D)J recombination [IDA]
- anatomical structure morphogenesis [TAS]
- base-excision repair [TAS]
- double-strand break repair [TAS]
- double-strand break repair via homologous recombination [TAS]
- lagging strand elongation [IBA]
- mitotic cell cycle [TAS]
- nucleotide-excision repair [IBA, TAS]
- nucleotide-excision repair, DNA gap filling [TAS]
- telomere maintenance [TAS]
- telomere maintenance via recombination [TAS]
- telomere maintenance via semi-conservative replication [TAS]
- transcription-coupled nucleotide-excision repair [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Co-crystal Structure
Interaction directly demonstrated at the atomic level by X-ray crystallography. Also used for NMR or Electron Microscopy (EM) structures. If there is no obvious bait-hit directionality to the interaction involving 3 or more proteins, then the co-crystallized proteins should be listed as a complex.
Publication
Structure of the UHRF1 Tandem Tudor Domain Bound to a Methylated Non-histone Protein, LIG1, Reveals Rules for Binding and Regulation.
The protein UHRF1 is crucial for DNA methylation maintenance. The tandem Tudor domain (TTD) of UHRF1 binds histone H3K9me2/3 with micromolar affinity, as well as unmethylated linker regions within UHRF1 itself, causing auto-inhibition. Recently, we showed that a methylated histone-like region of DNA ligase 1 (LIG1K126me2/me3) binds the UHRF1 TTD with nanomolar affinity, permitting UHRF1 recruitment to chromatin. Here we ... [more]
Throughput
- Low Throughput
Additional Notes
- cocrystal with LIG1 peptide
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| UHRF1 LIG1 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | - | BioGRID | - | |
| UHRF1 LIG1 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| LIG1 UHRF1 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| UHRF1 LIG1 | Co-localization Co-localization Interaction inferred from two proteins that co-localize in the cell by indirect immunofluorescence only when in addition, if one gene is deleted, the other protein becomes mis-localized. Also includes co-dependent association of proteins with promoter DNA in chromatin immunoprecipitation experiments. | Low | - | BioGRID | 2397303 | |
| LIG1 UHRF1 | Co-localization Co-localization Interaction inferred from two proteins that co-localize in the cell by indirect immunofluorescence only when in addition, if one gene is deleted, the other protein becomes mis-localized. Also includes co-dependent association of proteins with promoter DNA in chromatin immunoprecipitation experiments. | Low | - | BioGRID | - | |
| UHRF1 LIG1 | Protein-peptide Protein-peptide An interaction is detected between a protein and a peptide derived from an interaction partner. This includes phage display experiments. | Low | - | BioGRID | - | |
| UHRF1 LIG1 | Protein-peptide Protein-peptide An interaction is detected between a protein and a peptide derived from an interaction partner. This includes phage display experiments. | Low | - | BioGRID | - | |
| UHRF1 LIG1 | Protein-peptide Protein-peptide An interaction is detected between a protein and a peptide derived from an interaction partner. This includes phage display experiments. | Low | - | BioGRID | - | |
| UHRF1 LIG1 | Protein-peptide Protein-peptide An interaction is detected between a protein and a peptide derived from an interaction partner. This includes phage display experiments. | Low | - | BioGRID | - | |
| UHRF1 LIG1 | Reconstituted Complex Reconstituted Complex An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator. | Low | - | BioGRID | 2397306 | |
| UHRF1 LIG1 | Reconstituted Complex Reconstituted Complex An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator. | Low | - | BioGRID | - | |
| UHRF1 LIG1 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | BioGRID | 2693248 |
Curated By
- BioGRID