An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Calcineurin interacts with the serotonin transporter C-terminus to modulate its plasma membrane expression and serotonin uptake.

Seimandi M, Seyer P, Park CS, Vandermoere F, Chanrion B, Bockaert J, Mansuy IM, Marin P

Homeostasis of serotonergic transmission critically depends on the rate of serotonin reuptake via its plasma membrane transporter (SERT). SERT activity is tightly regulated by multiple mechanisms, including physical association with intracellular proteins and post-translational modifications, such as phosphorylation, but these mechanisms remain partially understood. Here, we show that SERT C-terminal domain recruits both the catalytic and regulatory subunits of the ... [more]

J. Neurosci. Oct. 09, 2013; 33(41);16189-99 [Pubmed: 24107951]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

SLC6A4

Gene Ontology Biological Process

Gene Ontology Molecular Function

Rab GTPase binding [IPI]actin filament binding [ISS]monoamine transmembrane transporter activity [IDA]protein binding [IPI]serotonin transmembrane transporter activity [IDA, IMP]serotonin:sodium symporter activity [IDA, IMP]

Gene Ontology Cellular Component

CALM1