BAIT

DAB1

AI956902, C630028C02Rik, scm, scr, scrambler, yot, RP23-356E21.1
disabled 1
Mus musculus
PREY

DOCK1

9130006G06Rik, AI854900, D630004B07Rik, mCG_125673
dedicator of cytokinesis 1
GO Process (2)
GO Function (1)
GO Component (3)

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Mus musculus

Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

Interaction between Dab1 and CrkII is promoted by Reelin signaling.

Chen K, Ochalski PG, Tran TS, Sahir N, Schubert M, Pramatarova A, Howell BW

Reelin-induced Dab1 tyrosine phosphorylation has been implicated in the regulation of neuronal positioning during brain development. The downstream consequences of Dab1 tyrosine phosphorylation are not fully understood, however. Here we identify CrkII, CrkL and Dock1 in complexes bound to tyrosine-phosphorylated Dab1, through mass spectrometry. The CrkII-Dab1 interaction requires tyrosine phosphorylation of Dab1 at residues 220 or 232 and is promoted ... [more]

J. Cell. Sci. Sep. 01, 2004; 117(Pt 19);4527-36 [Pubmed: 15316068]

Throughput

  • Low Throughput

Curated By

  • BioGRID