NCK2
Gene Ontology Biological Process
- T cell activation [NAS]
- axon guidance [TAS]
- epidermal growth factor receptor signaling pathway [TAS]
- negative regulation of cell proliferation [TAS]
- positive regulation of T cell proliferation [IMP]
- positive regulation of actin filament polymerization [IMP]
- positive regulation of transcription from RNA polymerase II promoter [IDA]
- regulation of epidermal growth factor-activated receptor activity [TAS]
- signal complex assembly [NAS]
- signal transduction [TAS]
Gene Ontology Molecular Function
CD3E
Gene Ontology Biological Process
- G-protein coupled receptor signaling pathway [TAS]
- T cell activation [NAS]
- T cell costimulation [TAS]
- T cell receptor signaling pathway [TAS]
- cell surface receptor signaling pathway [NAS]
- intracellular signal transduction [TAS]
- positive regulation of T cell proliferation [IMP]
- protein complex assembly [NAS]
- regulation of apoptotic process [NAS]
- regulation of immune response [TAS]
- signal complex assembly [TAS]
- transmembrane receptor protein tyrosine kinase signaling pathway [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Two-hybrid
Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.
Publication
A reference map of the human binary protein interactome.
Global insights into cellular organization and genome function require comprehensive understanding of the interactome networks that mediate genotype-phenotype relationships1,2. Here we present a human 'all-by-all' reference interactome map of human binary protein interactions, or 'HuRI'. With approximately 53,000 protein-protein interactions, HuRI has approximately four times as many such interactions as there are high-quality curated interactions from small-scale studies. The integration ... [more]
Throughput
- High Throughput
Additional Notes
- This human reference interactome (HuRI) was generated by performing nine two-hybrid screens with the high confidence interactions determined by pairwise verification by quadruplicate retesting and sequence confirmation. This HI-III-20 dataset contains over 52,000 PPIs involving more than 8,000 proteins.
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| NCK2 CD3E | Reconstituted Complex Reconstituted Complex An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator. | Low | - | BioGRID | - | |
| CD3E NCK2 | Reconstituted Complex Reconstituted Complex An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator. | Low | - | BioGRID | - | |
| CD3E NCK2 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | BioGRID | - |
Curated By
- BioGRID