Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

A human protein-protein interaction network: a resource for annotating the proteome.

Stelzl U, Worm U, Lalowski M, Haenig C, Brembeck FH, Goehler H, Stroedicke M, Zenkner M, Schoenherr A, Koeppen S, Timm J, Mintzlaff S, Abraham C, Bock N, Kietzmann S, Goedde A, Toksoez E, Droege A, Krobitsch S, Korn B, Birchmeier W, Lehrach H, Wanker EE

Protein-protein interaction maps provide a valuable framework for a better understanding of the functional organization of the proteome. To detect interacting pairs of human proteins systematically, a protein matrix of 4456 baits and 5632 preys was screened by automated yeast two-hybrid (Y2H) interaction mating. We identified 3186 mostly novel interactions among 1705 proteins, resulting in a large, highly connected network. ... [more]

Cell Sep. 23, 2005; 122(6);957-68 [Pubmed: 16169070]

Quantitative Score

2.0 [Confidence Score]

Throughput

High Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
SAP18 RPS3A	Proximity Label-MS Proximity Label-MS An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.	Kumari S (2024)	High	-	BioGRID	3755558

Curated By

BioGRID

Interaction Summary

RPS3A

Gene Ontology Biological Process

Gene Ontology Molecular Function

RNA binding [NAS]poly(A) RNA binding [IDA]protein binding [IPI]structural constituent of ribosome [IBA]

Gene Ontology Cellular Component

SAP18

Gene Ontology Biological Process

Gene Ontology Molecular Function

poly(A) RNA binding [IDA]protein binding [IPI]transcription corepressor activity [TAS]