Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

A human protein-protein interaction network: a resource for annotating the proteome.

Stelzl U, Worm U, Lalowski M, Haenig C, Brembeck FH, Goehler H, Stroedicke M, Zenkner M, Schoenherr A, Koeppen S, Timm J, Mintzlaff S, Abraham C, Bock N, Kietzmann S, Goedde A, Toksoez E, Droege A, Krobitsch S, Korn B, Birchmeier W, Lehrach H, Wanker EE

Protein-protein interaction maps provide a valuable framework for a better understanding of the functional organization of the proteome. To detect interacting pairs of human proteins systematically, a protein matrix of 4456 baits and 5632 preys was screened by automated yeast two-hybrid (Y2H) interaction mating. We identified 3186 mostly novel interactions among 1705 proteins, resulting in a large, highly connected network. ... [more]

Cell Sep. 23, 2005; 122(6);957-68 [Pubmed: 16169070]

Quantitative Score

4.0 [Confidence Score]

Throughput

High Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
ACTG1 PFN2	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Cho NH (2022)	High	-	BioGRID	3347444

Curated By

BioGRID

Interaction Summary

ACTG1

Gene Ontology Biological Process

Gene Ontology Molecular Function

identical protein binding [IPI]protein binding [IPI]structural constituent of cytoskeleton [IC]ubiquitin protein ligase binding [IPI]

Gene Ontology Cellular Component

PFN2

Gene Ontology Biological Process

Gene Ontology Molecular Function

actin monomer binding [IPI]adenyl-nucleotide exchange factor activity [IDA]phosphatidylinositol-4,5-bisphosphate binding [IDA]protein binding [IPI]