BAIT

ATKCO1

ATTPK1, CA2+ ACTIVATED OUTWARD RECTIFYING K+ CHANNEL 1, KCO1, MDF20.7, MDF20_7, TANDEM PORE K+ CHANNEL 1, TPK1, TWO PORE K CHANNEL, TWO PORE K CHANNEL 1, AT5G55630
two-pore potassium channel 1
Arabidopsis thaliana (Columbia)
PREY

GRF1

GENERAL REGULATORY FACTOR1-G-BOX FACTOR 14-3-3 HOMOLOG ISOFORM CHI, GF14 CHI, general regulatory factor 1, AT4G09000
14-3-3-like protein GF14 chi
GO Process (1)
GO Function (1)
GO Component (11)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Arabidopsis thaliana (Columbia)

Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

TPK1, a Ca(2+)-regulated Arabidopsis vacuole two-pore K(+) channel is activated by 14-3-3 proteins.

Latz A, Becker D, Hekman M, Mueller T, Beyhl D, Marten I, Eing C, Fischer A, Dunkel M, Bertl A, Rapp UR, Hedrich R

The vacuole represents a pivotal plant organelle for management of ion homeostasis, storage of proteins and solutes, as well as deposition of cytotoxic compounds. Ion channels, pumps and carriers in the vacuolar membrane under control of cytosolic factors provide for ionic and metabolic homeostasis between this storage organelle and the cytoplasm. Here we show that AtTPK1 (KCO1), a vacuolar membrane ... [more]

Plant J. Nov. 01, 2007; 52(3);449-59 [Pubmed: 17764516]

Throughput

  • Low Throughput

Additional Notes

  • Both proteins purified

Curated By

  • BioGRID