BAIT
PCBD1
DCOH, PCBD, PCD, PHS
pterin-4 alpha-carbinolamine dehydratase/dimerization cofactor of hepatocyte nuclear factor 1 alpha
GO Process (3)
GO Function (3)
GO Component (4)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
CACNA1C
CACH2, CACN2, CACNL1A1, CCHL1A1, CaV1.2, LQT8, TS
calcium channel, voltage-dependent, L type, alpha 1C subunit
GO Process (11)
GO Function (5)
GO Component (6)
Gene Ontology Biological Process
- axon guidance [TAS]
- calcium ion import [IBA]
- calcium ion transport into cytosol [TAS]
- calcium-mediated signaling using extracellular calcium source [TAS]
- cell communication by electrical coupling involved in cardiac conduction [TAS]
- energy reserve metabolic process [TAS]
- membrane depolarization during action potential [IBA]
- positive regulation of cytosolic calcium ion concentration [IDA]
- regulation of cardiac muscle contraction by regulation of the release of sequestered calcium ion [TAS]
- regulation of insulin secretion [TAS]
- small molecule metabolic process [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Two-hybrid
Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.
Publication
Homomeric and heteromeric interactions between wild-type and mutant phenylalanine hydroxylase subunits: evaluation of two-hybrid approaches for functional analysis of mutations causing hyperphenylalaninemia.
Phenylketonuria (PKU) is caused by mutations in the phenylalanine hydroxylase gene (PAH), while mutations in genes encoding the two enzymes (dihydropteridine reductase, DHPR, and pterin-4-alpha-carbinolamine dehydratase, PCD) required for recycling of its cofactor, tetrahydrobiopterin (BH(4)), cause other rarer disease forms of hyperphenylalaninemia. We have applied a yeast two-hybrid method, in which protein--protein interactions are measured by four reporter gene constructs, ... [more]
Mol. Genet. Metab. Jul. 01, 2001; 73(3);230-8 [Pubmed: 11461190]
Throughput
- Low Throughput
Curated By
- BioGRID