WTAP
Gene Ontology Biological Process
Gene Ontology Cellular Component
WT1
Gene Ontology Biological Process
- RNA splicing [ISS]
- adrenal cortex formation [ISS]
- adrenal gland development [IGI]
- branching involved in ureteric bud morphogenesis [IGI]
- camera-type eye development [ISS]
- cardiac muscle cell fate commitment [ISS]
- cellular response to cAMP [IEP]
- cellular response to gonadotropin stimulus [IDA]
- diaphragm development [ISS]
- epithelial cell differentiation [ISS]
- germ cell development [ISS]
- glomerular basement membrane development [IMP]
- glomerular visceral epithelial cell differentiation [IBA, ISS]
- glomerulus development [IGI]
- gonad development [ISS]
- heart development [IGI]
- kidney development [IGI]
- male genitalia development [ISS]
- male gonad development [IEP]
- mesenchymal to epithelial transition [ISS]
- metanephric S-shaped body morphogenesis [IGI]
- metanephric epithelium development [IEP]
- metanephric mesenchyme development [ISS]
- negative regulation of apoptotic process [IGI]
- negative regulation of cell growth [IDA]
- negative regulation of cell proliferation [IDA]
- negative regulation of female gonad development [ISS]
- negative regulation of metanephric glomerular mesangial cell proliferation [ISS]
- negative regulation of transcription from RNA polymerase II promoter [IDA]
- negative regulation of transcription, DNA-templated [IDA]
- negative regulation of translation [IDA]
- positive regulation of apoptotic process [IDA]
- positive regulation of heart growth [ISS]
- positive regulation of male gonad development [ISS]
- positive regulation of metanephric ureteric bud development [ISS]
- positive regulation of transcription from RNA polymerase II promoter [ISS]
- positive regulation of transcription, DNA-templated [IDA, IGI]
- posterior mesonephric tubule development [ISS]
- regulation of organ formation [ISS]
- regulation of transcription from RNA polymerase II promoter [ISS]
- regulation of transcription, DNA-templated [ISS, NAS]
- sex determination [IDA]
- thorax and anterior abdomen determination [ISS]
- tissue development [ISS]
- ureteric bud development [ISS]
- vasculogenesis [ISS]
- visceral serous pericardium development [IGI]
Gene Ontology Molecular Function- C2H2 zinc finger domain binding [IPI]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [ISS]
- protein binding [IPI]
- sequence-specific DNA binding [IDA]
- sequence-specific DNA binding transcription factor activity [ISS, NAS]
- transcription regulatory region DNA binding [IDA]
- zinc ion binding [IDA]
- C2H2 zinc finger domain binding [IPI]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [ISS]
- protein binding [IPI]
- sequence-specific DNA binding [IDA]
- sequence-specific DNA binding transcription factor activity [ISS, NAS]
- transcription regulatory region DNA binding [IDA]
- zinc ion binding [IDA]
Gene Ontology Cellular Component
- cytoplasm [IBA, ISS]
- nuclear speck [IDA]
- nucleoplasm [IDA]
- nucleus [IDA]
Two-hybrid
Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.
Publication
A reference map of the human binary protein interactome.
Global insights into cellular organization and genome function require comprehensive understanding of the interactome networks that mediate genotype-phenotype relationships1,2. Here we present a human 'all-by-all' reference interactome map of human binary protein interactions, or 'HuRI'. With approximately 53,000 protein-protein interactions, HuRI has approximately four times as many such interactions as there are high-quality curated interactions from small-scale studies. The integration ... [more]
Throughput
- High Throughput
Additional Notes
- This human reference interactome (HuRI) was generated by performing nine two-hybrid screens with the high confidence interactions determined by pairwise verification by quadruplicate retesting and sequence confirmation. This HI-III-20 dataset contains over 52,000 PPIs involving more than 8,000 proteins.
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| WT1 WTAP | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| WTAP WT1 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| WTAP WT1 | Reconstituted Complex Reconstituted Complex An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator. | Low | - | BioGRID | - | |
| WT1 WTAP | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | BioGRID | - |
Curated By
- BioGRID