Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Differential regulation of glucocorticoid receptor transcriptional activation via AF-1-associated proteins.

Hittelman AB, Burakov D, Iniguez-Lluhi JA, Freedman LP, Garabedian MJ

The hormone-activated glucocorticoid receptor (GR), through its N- and C-terminal transcriptional activation functions AF-1 and AF-2, controls the transcription of target genes presumably through interaction(s) with transcriptional regulatory factors. Utilizing a modified yeast two-hybrid approach, we have identified the tumor susceptibility gene 101 (TSG101) and the vitamin D receptor-interacting protein 150 (DRIP150) as proteins that interact specifically with a functional ... [more]

EMBO J. Oct. 01, 1999; 18(19);5380-8 [Pubmed: 10508170]

Throughput

  • Low Throughput

Curated By

  • BioGRID