SMO
Gene Ontology Biological Process
- canonical Wnt signaling pathway [IBA]
- cellular response to cholesterol [ISS]
- detection of cell density by contact stimulus involved in contact inhibition [IMP]
- determination of left/right asymmetry in lateral mesoderm [ISS]
- forebrain morphogenesis [ISS]
- heart looping [ISS]
- mesenchymal to epithelial transition involved in metanephric renal vesicle formation [ISS]
- midgut development [ISS]
- negative regulation of DNA binding [ISS]
- negative regulation of gene expression [ISS]
- non-canonical Wnt signaling pathway [IBA]
- positive regulation of branching involved in ureteric bud morphogenesis [ISS]
- positive regulation of gene expression [ISS]
- positive regulation of hh target transcription factor activity [ISS]
- regulation of heart morphogenesis [ISS]
- renal system development [IEP]
- smoothened signaling pathway [IEP, IMP]
- somite development [ISS]
- ventral midline determination [ISS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
HERC4
Gene Ontology Biological Process
Gene Ontology Molecular Function
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
HERC4 exerts an anti-tumor role through destabilizing the oncoprotein Smo.
The GPCR-like transmembrane protein Smoothened (Smo) is an indispensable transducer in Hedgehog (Hh) pathway, its hyperactivation leads to several human cancers, including non-small cell lung cancer (NSCLC). The mechanism governing Smo stability still remains elusive. Here, we perform a modifier screening in Drosophila, and find that the E3 ligase dHerc4 degrades dSmo. Depletion of dherc4 increases dSmo protein and activates ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| HERC4 SMO | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - |
Curated By
- BioGRID