BAIT

SYT1

P65, SVP65, SYT
synaptotagmin I
Homo sapiens
PREY

ATG16L1

APG16L, ATG16A, ATG16L, IBD10, WDR30, hCG_1817841
autophagy related 16-like 1 (S. cerevisiae)
Autophagy Project
UPS Project
GO Process (3)
GO Function (2)
GO Component (3)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Atg16L1, an essential factor for canonical autophagy, participates in hormone secretion from PC12 cells independently of autophagic activity.

Ishibashi K, Uemura T, Waguri S, Fukuda M

Autophagy is a bulk degradation system in all eukaryotic cells and regulates a variety of biological activities in higher eukaryotes. Recently involvement of autophagy in the regulation of the secretory pathway has also been reported, but the molecular mechanism linking autophagy with the secretory pathway remains largely unknown. Here we show that Atg16L1, an essential protein for canonical autophagy, is ... [more]

Mol. Biol. Cell Aug. 01, 2012; 23(16);3193-202 [Pubmed: 22740627]

Throughput

  • Low Throughput

Curated By

  • BioGRID