An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

Identification of anaplastic lymphoma kinase as a receptor for the growth factor pleiotrophin.

Stoica GE, Kuo A, Aigner A, Sunitha I, Souttou B, Malerczyk C, Caughey DJ, Wen D, Karavanov A, Riegel AT, Wellstein A

Pleiotrophin (PTN) is a secreted growth factor that induces neurite outgrowth and is mitogenic for fibroblasts, epithelial, and endothelial cells. During tumor growth PTN can serve as an angiogenic factor and drive tumor invasion and metastasis. To identify a receptor for PTN, we panned a phage display human cDNA library against immobilized PTN protein as a bait. From this we ... [more]

J. Biol. Chem. May. 18, 2001; 276(20);16772-9 [Pubmed: 11278720]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

ALK

Gene Ontology Biological Process

Gene Ontology Molecular Function

NF-kappaB-inducing kinase activity [TAS]protein binding [IPI]protein tyrosine kinase activity [IDA]transmembrane receptor protein tyrosine kinase activity [IDA, TAS]

Gene Ontology Cellular Component

PTN