An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Impaired plasma membrane localization of ubiquitin ligase complex underlies 3-M syndrome development.

Wang P, Yan F, Li Z, Yu Y, Parnell SE, Xiong Y

3-M primordial dwarfism is an inherited disease characterized by severe pre- and postnatal growth retardation and by mutually exclusive mutations in three genes, CUL7, OBSL1, and CCDC8. The mechanism underlying 3-M dwarfism is not clear. We showed here that CCDC8, derived from a retrotransposon Gag protein in placental mammals, exclusively localized on the plasma membrane and was phosphorylated by CK2 ... [more]

J. Clin. Invest. Jul. 25, 2019; 130();4393-4407 [Pubmed: 31343991]

Throughput

High Throughput

Curated By

BioGRID

Interaction Summary

CCDC8

Gene Ontology Biological Process

Gene Ontology Cellular Component

TUBB2A