Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

SUMOylation of Csk Negatively Modulates its Tumor Suppressor Function.

Cui N, Liu T, Guo Y, Dou J, Yang Q, Zhang H, Chen R, Wang Y, Zhao X, Yu J, Huang J

Csk, a non-receptor tyrosine kinase, serves as an indispensable negative regulator of the Src family kinases (SFKs). However, little is known about regulation of Csk expression so far. SUMOylation, a reversible post-translational modification, has been shown to regulate many biological processes especially in tumor progression. Here we report that Csk is covalently modified by SUMO1 at lysine 53 (K53) both ... [more]

Neoplasia Dec. 01, 2018; 21(7);676-688 [Pubmed: 31125786]

Throughput

  • Low Throughput

Curated By

  • BioGRID