Co-purification

An interaction is inferred from the identification of two or more protein subunits in a purified protein complex, as obtained by classical biochemical fractionation or affinity purification and one or more additional fractionation steps.

Publication

Association of spectrin with desmin intermediate filaments.

Langley RC, Cohen CM

The association of erythrocyte spectrin with desmin filaments was investigated using two in vitro assays. The ability of spectrin to promote the interaction of desmin filaments with membranes was investigated by electron microscopy of desmin filament-erythrocyte inside-out vesicle preparations. Desmin filaments bound to erythrocyte inside-out vesicles in a spectrin-dependent manner, demonstrating that spectrin is capable of mediating the association of ... [more]

J. Cell. Biochem. Jan. 01, 1986; 30(2);101-9 [Pubmed: 2939097]

Throughput

  • Low Throughput

Curated By

  • BioGRID