BAIT
PLEKHA4
PEPP1
pleckstrin homology domain containing, family A (phosphoinositide binding specific) member 4
GO Process (0)
GO Function (1)
GO Component (0)
Gene Ontology Molecular Function
Homo sapiens
PREY
APBB1
FE65, MGC:9072, RIR
amyloid beta (A4) precursor protein-binding, family B, member 1 (Fe65)
GO Process (10)
GO Function (6)
GO Component (6)
Gene Ontology Biological Process
- axonogenesis [NAS]
- cell cycle arrest [ISS]
- cellular response to DNA damage stimulus [IDA]
- histone H4 acetylation [ISS]
- negative regulation of cell growth [ISS]
- negative regulation of thymidylate synthase biosynthetic process [ISS]
- positive regulation of apoptotic process [IDA]
- positive regulation of transcription, DNA-templated [IDA]
- regulation of transcription, DNA-templated [ISS]
- signal transduction [NAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
PLEKHA4/kramer Attenuates Dishevelled Ubiquitination to Modulate Wnt and Planar Cell Polarity Signaling.
Wnt signaling pathways direct key physiological decisions in development. Here, we establish a role for a pleckstrin homology domain-containing protein, PLEKHA4, as a modulator of signaling strength in Wnt-receiving cells. PLEKHA4 oligomerizes into clusters at PI(4,5)P2-rich regions of the plasma membrane and recruits the Cullin-3 (CUL3) E3 ubiquitin ligase substrate adaptor Kelch-like protein 12 (KLHL12) to these assemblies. This recruitment decreases ... [more]
Cell Rep Dec. 14, 2018; 27(7);2157-2170.e8 [Pubmed: 31091453]
Throughput
- High Throughput
Additional Notes
- assayed using SILAC IP (stable isotope labeling by amino acids in cell culture-based quantitative immunoprecipitation)
- only hits identified in both pull-downs have been included
Curated By
- BioGRID