Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Dynein light chain interacts with NRF-1 and EWG, structurally and functionally related transcription factors from humans and drosophila.

Herzig RP, Andersson U, Scarpulla RC

Nuclear respiratory factor-1 is a transcriptional activator that has been implicated in the nuclear control of respiratory chain expression. Yeast two-hybrid screens were performed to identify proteins that physically interact with nuclear respiratory factor-1. Saturation screening of both mouse embryo and mouse testis libraries yielded 14 independent clones, all of which represented two different isoforms of dynein light chain. In ... [more]

J. Cell. Sci. Dec. 01, 2000; 113(0);4263-73 [Pubmed: 11069771]

Throughput

  • Low Throughput

Curated By

  • BioGRID