An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

The N-terminal end of nebulin interacts with tropomodulin at the pointed ends of the thin filaments.

McElhinny AS, Kolmerer B, Fowler VM, Labeit S, Gregorio CC

Strict regulation of actin thin filament length is critical for the proper functioning of sarcomeres, the basic contractile units of myofibrils. It has been hypothesized that a molecular template works with actin filament capping proteins to regulate thin filament lengths. Nebulin is a giant protein ( approximately 800 kDa) in skeletal muscle that has been proposed to act as a ... [more]

J. Biol. Chem. Jan. 05, 2001; 276(1);583-92 [Pubmed: 11016930]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

NEB

Gene Ontology Biological Process

Gene Ontology Molecular Function

protein binding [IPI]structural constituent of muscle [TAS]

Gene Ontology Cellular Component

TMOD1