BAIT
PLEKHA4
PEPP1
pleckstrin homology domain containing, family A (phosphoinositide binding specific) member 4
GO Process (0)
GO Function (1)
GO Component (0)
Gene Ontology Molecular Function
Homo sapiens
PREY
LMNA
CDCD1, CDDC, CMD1A, CMT2B1, EMD2, FPL, FPLD, FPLD2, HGPS, IDC, LDP1, LFP, LGMD1B, LMN1, LMNC, LMNL1, PRO1, RP11-54H19.1
lamin A/C
GO Process (14)
GO Function (2)
GO Component (9)
Gene Ontology Biological Process
- activation of signaling protein activity involved in unfolded protein response [TAS]
- apoptotic process [TAS]
- cellular component disassembly involved in execution phase of apoptosis [TAS]
- cellular protein metabolic process [TAS]
- cellular response to hypoxia [IEP]
- endoplasmic reticulum unfolded protein response [TAS]
- establishment or maintenance of microtubule cytoskeleton polarity [ISS]
- mitotic cell cycle [TAS]
- mitotic nuclear envelope disassembly [TAS]
- mitotic nuclear envelope reassembly [TAS]
- muscle organ development [IMP]
- positive regulation of cell aging [IDA]
- protein localization to nucleus [ISS]
- regulation of cell migration [ISS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
PLEKHA4/kramer Attenuates Dishevelled Ubiquitination to Modulate Wnt and Planar Cell Polarity Signaling.
Wnt signaling pathways direct key physiological decisions in development. Here, we establish a role for a pleckstrin homology domain-containing protein, PLEKHA4, as a modulator of signaling strength in Wnt-receiving cells. PLEKHA4 oligomerizes into clusters at PI(4,5)P2-rich regions of the plasma membrane and recruits the Cullin-3 (CUL3) E3 ubiquitin ligase substrate adaptor Kelch-like protein 12 (KLHL12) to these assemblies. This recruitment decreases ... [more]
Cell Rep Dec. 14, 2018; 27(7);2157-2170.e8 [Pubmed: 31091453]
Throughput
- High Throughput
Additional Notes
- assayed using SILAC IP (stable isotope labeling by amino acids in cell culture-based quantitative immunoprecipitation)
- only hits identified in both pull-downs have been included
Curated By
- BioGRID