BAIT
PLEKHA4
PEPP1
pleckstrin homology domain containing, family A (phosphoinositide binding specific) member 4
GO Process (0)
GO Function (1)
GO Component (0)
Gene Ontology Molecular Function
Homo sapiens
PREY
PPP1CC
PP-1G, PP1C, PPP1G
protein phosphatase 1, catalytic subunit, gamma isozyme
GO Process (9)
GO Function (6)
GO Component (9)
Gene Ontology Biological Process
- circadian regulation of gene expression [ISS]
- entrainment of circadian clock by photoperiod [ISS]
- mitotic cell cycle [TAS]
- negative regulation of transforming growth factor beta receptor signaling pathway [TAS]
- protein dephosphorylation [IMP, ISS]
- regulation of circadian rhythm [IMP]
- small molecule metabolic process [TAS]
- transforming growth factor beta receptor signaling pathway [TAS]
- triglyceride catabolic process [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
PLEKHA4/kramer Attenuates Dishevelled Ubiquitination to Modulate Wnt and Planar Cell Polarity Signaling.
Wnt signaling pathways direct key physiological decisions in development. Here, we establish a role for a pleckstrin homology domain-containing protein, PLEKHA4, as a modulator of signaling strength in Wnt-receiving cells. PLEKHA4 oligomerizes into clusters at PI(4,5)P2-rich regions of the plasma membrane and recruits the Cullin-3 (CUL3) E3 ubiquitin ligase substrate adaptor Kelch-like protein 12 (KLHL12) to these assemblies. This recruitment decreases ... [more]
Cell Rep Dec. 14, 2018; 27(7);2157-2170.e8 [Pubmed: 31091453]
Throughput
- High Throughput
Additional Notes
- assayed using SILAC IP (stable isotope labeling by amino acids in cell culture-based quantitative immunoprecipitation)
- only hits identified in both pull-downs have been included
Curated By
- BioGRID