MAFK
Gene Ontology Biological Process
Gene Ontology Cellular Component
NFE2L2
Gene Ontology Biological Process
- cellular response to fluid shear stress [IDA]
- cellular response to hydrogen peroxide [IMP]
- cellular response to laminar fluid shear stress [IMP]
- cellular response to tumor necrosis factor [IMP]
- negative regulation of endothelial cell apoptotic process [IMP]
- negative regulation of hydrogen peroxide-induced cell death [IGI]
- negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway [IMP]
- positive regulation of gene expression [IGI]
- positive regulation of transcription from RNA polymerase II promoter [IC, IDA, IMP]
- positive regulation of transcription from RNA polymerase II promoter in response to stress [IMP]
- proteasomal ubiquitin-independent protein catabolic process [IDA]
- proteasome-mediated ubiquitin-dependent protein catabolic process [IDA]
- protein ubiquitination [IDA]
- transcription from RNA polymerase II promoter [TAS]
Gene Ontology Molecular Function- DNA binding [IDA]
- RNA polymerase II activating transcription factor binding [IPI]
- protein binding [IPI]
- protein domain specific binding [IPI]
- sequence-specific DNA binding transcription factor activity [IDA]
- transcription regulatory region DNA binding [TAS]
- transcription regulatory region sequence-specific DNA binding [TAS]
- DNA binding [IDA]
- RNA polymerase II activating transcription factor binding [IPI]
- protein binding [IPI]
- protein domain specific binding [IPI]
- sequence-specific DNA binding transcription factor activity [IDA]
- transcription regulatory region DNA binding [TAS]
- transcription regulatory region sequence-specific DNA binding [TAS]
Gene Ontology Cellular Component
Reconstituted Complex
An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.
Publication
Transcriptional regulation of the antioxidant response element. Activation by Nrf2 and repression by MafK.
The antioxidant response element (ARE) mediates the transcriptional activation of many genes encoding phase II drug-metabolizing enzymes in response to oxidative stress. Recent studies using knockout mice suggest that NF-E2-related factor 2 (Nrf2), along with small Maf proteins, binds and activates the ARE. In this study, using in vitro binding assays, Nrf2/MafK heterodimers were found to interact with high affinity ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| MAFK NFE2L2 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | 0.8917 | BioGRID | 3263407 | |
| MAFK NFE2L2 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | 0.9995 | BioGRID | 3097597 | |
| MAFK NFE2L2 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| NFE2L2 MAFK | FRET FRET An interaction is inferred when close proximity of interaction partners is detected by fluorescence resonance energy transfer between pairs of fluorophore-labeled molecules, such as occurs between CFP (donor) and YFP (acceptor) fusion proteins. | Low | - | BioGRID | 3012600 | |
| NFE2L2 MAFK | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | High | - | BioGRID | 2716611 | |
| MAFK NFE2L2 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | High | - | BioGRID | - |
Curated By
- BioGRID