STX1A
Gene Ontology Biological Process
- cellular protein metabolic process [TAS]
- energy reserve metabolic process [TAS]
- glutamate secretion [TAS]
- intracellular protein transport [IBA]
- neurotransmitter secretion [TAS]
- regulation of insulin secretion [TAS]
- secretion by cell [IDA]
- small molecule metabolic process [TAS]
- synaptic transmission [TAS]
- synaptic vesicle fusion to presynaptic membrane [IBA]
- vesicle docking [IBA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
CPLX2
Gene Ontology Biological Process
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
A conformational switch in syntaxin during exocytosis: role of munc18.
Syntaxin 1, an essential protein in synaptic membrane fusion, contains a helical autonomously folded N-terminal domain, a C-terminal SNARE motif and a transmembrane region. The SNARE motif binds to synaptobrevin and SNAP-25 to assemble the core complex, whereas almost the entire cytoplasmic sequence participates in a complex with munc18-1, a neuronal Sec1 homolog. We now demonstrate by NMR spectroscopy that, ... [more]
Throughput
- Low Throughput
Curated By
- BioGRID