BAIT
PLEKHA4
PEPP1
pleckstrin homology domain containing, family A (phosphoinositide binding specific) member 4
GO Process (0)
GO Function (1)
GO Component (0)
Gene Ontology Molecular Function
Homo sapiens
PREY
SCRIB
CRIB1, SCRB1, SCRIB1, Vartul
scribbled planar cell polarity protein
GO Process (13)
GO Function (1)
GO Component (9)
Gene Ontology Biological Process
- activation of Rac GTPase activity [IMP]
- apoptotic process involved in morphogenesis [IMP]
- cell migration [IMP]
- cell proliferation [IDA]
- establishment of apical/basal cell polarity [IMP]
- mammary gland duct morphogenesis [ISS]
- negative regulation of mitotic cell cycle [IDA]
- neural tube closure [IMP]
- positive chemotaxis [IMP]
- positive regulation of apoptotic process [IMP]
- positive regulation of receptor recycling [IMP]
- protein localization to adherens junction [IMP]
- single organismal cell-cell adhesion [IGI]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
PLEKHA4/kramer Attenuates Dishevelled Ubiquitination to Modulate Wnt and Planar Cell Polarity Signaling.
Wnt signaling pathways direct key physiological decisions in development. Here, we establish a role for a pleckstrin homology domain-containing protein, PLEKHA4, as a modulator of signaling strength in Wnt-receiving cells. PLEKHA4 oligomerizes into clusters at PI(4,5)P2-rich regions of the plasma membrane and recruits the Cullin-3 (CUL3) E3 ubiquitin ligase substrate adaptor Kelch-like protein 12 (KLHL12) to these assemblies. This recruitment decreases ... [more]
Cell Rep Dec. 14, 2018; 27(7);2157-2170.e8 [Pubmed: 31091453]
Throughput
- High Throughput
Additional Notes
- assayed using SILAC IP (stable isotope labeling by amino acids in cell culture-based quantitative immunoprecipitation)
- only hits identified in both pull-downs have been included
Curated By
- BioGRID