BAIT
PLEKHA4
PEPP1
pleckstrin homology domain containing, family A (phosphoinositide binding specific) member 4
GO Process (0)
GO Function (1)
GO Component (0)
Gene Ontology Molecular Function
Homo sapiens
PREY
MINK1
B55, MAP4K6, MINK, YSK2, ZC3, hMINK, hMINKbeta
misshapen-like kinase 1
GO Process (14)
GO Function (5)
GO Component (4)
Gene Ontology Biological Process
- JNK cascade [TAS]
- actin cytoskeleton reorganization [IMP]
- dendrite morphogenesis [ISS]
- intracellular signal transduction [ISS]
- multicellular organismal development [ISS]
- positive regulation of JNK cascade [IMP]
- protein autophosphorylation [IDA]
- protein phosphorylation [ISS]
- regulation of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate selective glutamate receptor activity [ISS]
- regulation of cell migration [IMP]
- regulation of cell-cell adhesion [IMP]
- regulation of cell-matrix adhesion [IMP]
- response to stress [ISS]
- synaptic transmission [ISS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
PLEKHA4/kramer Attenuates Dishevelled Ubiquitination to Modulate Wnt and Planar Cell Polarity Signaling.
Wnt signaling pathways direct key physiological decisions in development. Here, we establish a role for a pleckstrin homology domain-containing protein, PLEKHA4, as a modulator of signaling strength in Wnt-receiving cells. PLEKHA4 oligomerizes into clusters at PI(4,5)P2-rich regions of the plasma membrane and recruits the Cullin-3 (CUL3) E3 ubiquitin ligase substrate adaptor Kelch-like protein 12 (KLHL12) to these assemblies. This recruitment decreases ... [more]
Cell Rep Dec. 14, 2018; 27(7);2157-2170.e8 [Pubmed: 31091453]
Throughput
- High Throughput
Additional Notes
- assayed using SILAC IP (stable isotope labeling by amino acids in cell culture-based quantitative immunoprecipitation)
- only hits identified in both pull-downs have been included
Curated By
- BioGRID