BAIT

RAD18

RNF73
RAD18 E3 ubiquitin protein ligase
UPS Project
GO Process (1)
GO Function (5)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens
PREY

PARP1

ADPRT, ADPRT 1, ADPRT1, ARTD1, PARP, PARP-1, PPOL, pADPRT-1, RP11-125A15.2
poly (ADP-ribose) polymerase 1
Homo sapiens

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

SETD1A Methyltransferase Is Physically and Functionally Linked to the DNA Damage Repair Protein RAD18.

Alsulami M, Munawar N, Dillon E, Oliviero G, Wynne K, Alsolami M, Moss C, O Gaora P, O'Meara F, Cotter D, Cagney G

SETD1A is a SET domain-containing methyltransferase involved in epigenetic regulation of transcription. It is the main catalytic component of a multiprotein complex that methylates lysine 4 of histone H3, a histone mark associated with gene activation. In humans, six related protein complexes with partly nonredundant cellular functions share several protein subunits but are distinguished by unique catalytic SET-domain proteins. We ... [more]

Mol. Cell Proteomics Dec. 01, 2018; 18(7);1428-1436 [Pubmed: 31076518]

Throughput

  • High Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
PARP1 RAD18
Proximity Label-MS
Proximity Label-MS

An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

High18.67BioGRID
2998653

Curated By

  • BioGRID