Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

hLodestar/HuF2 interacts with CDC5L and is involved in pre-mRNA splicing.

Leonard D, Ajuh P, Lamond AI, Legerski RJ

hLodestar/HuF2 belongs to the SNF2 family of proteins. This family of proteins has been shown to play a critical role in altering protein-DNA interactions in a variety of cellular contexts. We have identified an unexpected interaction between hLodestar/HuF2 and CDC5L in both the yeast two-hybrid system and HeLa nuclear extract. CDC5L is a well-characterized pre-mRNA splicing factor in yeast and ... [more]

Biochem. Biophys. Res. Commun. Sep. 05, 2003; 308(4);793-801 [Pubmed: 12927788]

Throughput

  • Low Throughput

Curated By

  • BioGRID