BAIT
MIB1
DIP-1, DIP1, LVNC7, MIB, ZZANK2, ZZZ6
mindbomb E3 ubiquitin protein ligase 1
GO Process (3)
GO Function (2)
GO Component (3)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Homo sapiens
PREY
ARHGAP35
GRF-1, GRLF1, P190-A, P190A, p190ARhoGAP, p190RhoGAP
Rho GTPase activating protein 35
GO Process (6)
GO Function (5)
GO Component (2)
Gene Ontology Biological Process
- axon guidance [TAS]
- negative regulation of transcription from RNA polymerase II promoter [IDA]
- negative regulation of transcription, DNA-templated [IDA]
- positive regulation of Rho GTPase activity [TAS]
- regulation of small GTPase mediated signal transduction [TAS]
- small GTPase mediated signal transduction [TAS]
Gene Ontology Molecular Function- DNA binding [TAS]
- RNA polymerase II regulatory region sequence-specific DNA binding [IDA]
- RNA polymerase II transcription regulatory region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription [IDA]
- Rho GTPase activator activity [TAS]
- transcription corepressor activity [TAS]
- DNA binding [TAS]
- RNA polymerase II regulatory region sequence-specific DNA binding [IDA]
- RNA polymerase II transcription regulatory region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription [IDA]
- Rho GTPase activator activity [TAS]
- transcription corepressor activity [TAS]
Homo sapiens
Proximity Label-MS
An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.
Publication
Proximity interactions of the ubiquitin ligase Mind bomb 1 reveal a role in regulation of epithelial polarity complex proteins.
MIB1 belongs to the RING domain containing family of E3 ubiquitin ligases. In vertebrates, MIB1 plays an essential role in activation of Notch signaling during development, through the ubiquitination and endocytosis of Notch ligands. More recently, Notch independent functions for MIB1 have been described in centriole homeostasis, dendritic spine outgrowth and directional cell migration. Here we use proximity-dependent biotin identification ... [more]
Sci Rep Dec. 28, 2018; 9(1);12471 [Pubmed: 31462741]
Throughput
- High Throughput
Additional Notes
- assayed using BioID (proximity-dependent biotin identification)
Curated By
- BioGRID