BAIT
SHC1
P66shc
SHC (Src homology 2 domain containing) transforming protein 1
GO Process (25)
GO Function (12)
GO Component (5)
Gene Ontology Biological Process
- MAPK cascade [ISO]
- actin cytoskeleton organization [IMP]
- actin cytoskeleton reorganization [ISO]
- activation of MAPK activity [IMP, ISO]
- aging [IPI]
- angiogenesis [ISO]
- cellular response to insulin stimulus [IEP]
- epidermal growth factor receptor signaling pathway [IBA, ISO]
- heart development [ISO]
- insulin receptor signaling pathway [IDA, IMP]
- neuron differentiation [IMP]
- neuron projection development [IMP]
- organ regeneration [IEP]
- positive regulation of DNA replication [IMP]
- positive regulation of smooth muscle cell proliferation [IMP]
- positive regulation of vasoconstriction [IMP]
- regulation of cell proliferation [ISO]
- response to glucocorticoid [IEP]
- response to hydrogen peroxide [IEP]
- response to hypoxia [IEP]
- response to insulin [IEP]
- response to nicotine [IEP]
- response to organic cyclic compound [IEP]
- response to toxic substance [IEP]
- single organismal cell-cell adhesion [ISO]
Gene Ontology Molecular Function- ephrin receptor binding [ISO]
- epidermal growth factor receptor binding [IPI]
- insulin receptor binding [ISO]
- insulin-like growth factor receptor binding [ISO]
- neurotrophin TRKA receptor binding [ISO]
- phosphoprotein binding [IPI]
- phosphotyrosine binding [IPI]
- protein binding [IPI]
- protein complex binding [IPI]
- protein kinase binding [IBA]
- protein phosphatase 2A binding [IDA, IPI]
- receptor tyrosine kinase binding [IPI, ISO]
- ephrin receptor binding [ISO]
- epidermal growth factor receptor binding [IPI]
- insulin receptor binding [ISO]
- insulin-like growth factor receptor binding [ISO]
- neurotrophin TRKA receptor binding [ISO]
- phosphoprotein binding [IPI]
- phosphotyrosine binding [IPI]
- protein binding [IPI]
- protein complex binding [IPI]
- protein kinase binding [IBA]
- protein phosphatase 2A binding [IDA, IPI]
- receptor tyrosine kinase binding [IPI, ISO]
Gene Ontology Cellular Component
Rattus norvegicus
PREY
NTRK2
RATTRKB1, TRKB1, Tkrb, trk-B, trkB
neurotrophic tyrosine kinase, receptor, type 2
GO Process (44)
GO Function (8)
GO Component (24)
Gene Ontology Biological Process
- aging [IEP]
- brain-derived neurotrophic factor receptor signaling pathway [IDA, IMP, ISO]
- calcium-mediated signaling using intracellular calcium source [ISO]
- cellular response to brain-derived neurotrophic factor stimulus [IEP]
- cellular response to nerve growth factor stimulus [IEP]
- cellular response to tumor necrosis factor [IEP]
- central nervous system neuron development [ISO, ISS]
- cerebral cortex development [ISO, ISS]
- feeding behavior [ISO]
- glutamate secretion [ISO]
- inflammatory response [IEP]
- learning [ISO, ISS]
- long-term memory [IDA]
- long-term synaptic potentiation [ISO]
- mechanoreceptor differentiation [ISO]
- negative regulation of anoikis [ISO]
- negative regulation of neuron apoptotic process [IMP]
- neuromuscular junction development [ISO]
- neuron differentiation [ISO, ISS]
- neuron migration [ISO, ISS]
- neurotrophin signaling pathway [IDA, IMP, ISO]
- oligodendrocyte differentiation [ISO]
- peptidyl-tyrosine phosphorylation [IMP, ISO]
- peripheral nervous system neuron development [ISO]
- positive regulation of MAPK cascade [IMP]
- positive regulation of axonogenesis [IMP]
- positive regulation of cell proliferation [IDA]
- positive regulation of gene expression [ISO, ISS]
- positive regulation of glucocorticoid receptor signaling pathway [IGI]
- positive regulation of neuron projection development [ISO, ISS]
- positive regulation of peptidyl-serine phosphorylation [ISO]
- positive regulation of phosphatidylinositol 3-kinase signaling [IMP]
- positive regulation of synaptic transmission, glutamatergic [IMP]
- protein autophosphorylation [IDA, IMP]
- regulation of MAPK cascade [IDA]
- regulation of Rac GTPase activity [ISO, ISS]
- regulation of dendrite development [IMP]
- regulation of metabolic process [ISO]
- regulation of neurotransmitter secretion [IDA]
- regulation of protein kinase B signaling [ISO]
- response to auditory stimulus [IEP]
- response to light stimulus [IEP]
- retinal rod cell development [ISO]
- vasculogenesis [ISO]
Gene Ontology Molecular Function- brain-derived neurotrophic factor binding [IDA, IMP, ISO]
- brain-derived neurotrophic factor-activated receptor activity [IDA, ISO]
- ephrin receptor binding [IPI]
- neurotrophin binding [IDA, ISO, ISS]
- neurotrophin receptor activity [IDA, IMP]
- protein binding [IPI]
- protein homodimerization activity [IDA]
- transmembrane receptor protein tyrosine kinase activity [IMP]
- brain-derived neurotrophic factor binding [IDA, IMP, ISO]
- brain-derived neurotrophic factor-activated receptor activity [IDA, ISO]
- ephrin receptor binding [IPI]
- neurotrophin binding [IDA, ISO, ISS]
- neurotrophin receptor activity [IDA, IMP]
- protein binding [IPI]
- protein homodimerization activity [IDA]
- transmembrane receptor protein tyrosine kinase activity [IMP]
Gene Ontology Cellular Component
- Golgi membrane [TAS]
- axon [IDA]
- axon terminus [IDA]
- cell [ISO]
- cell surface [IDA]
- cytoplasm [IDA]
- cytosol [ISO]
- dendrite [IDA]
- dendritic spine [IDA]
- endosome [ISO]
- excitatory synapse [IDA]
- growth cone [IDA]
- integral component of plasma membrane [IDA, ISO]
- intracellular [ISO]
- neuronal cell body [IDA]
- neuronal postsynaptic density [ISO]
- perikaryon [IDA]
- plasma membrane [IDA, ISO, TAS]
- postsynaptic density [IDA]
- postsynaptic membrane [ISO]
- presynaptic active zone [IDA]
- receptor complex [ISO]
- rough endoplasmic reticulum [IDA]
- terminal bouton [ISO]
Rattus norvegicus
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Brain-derived neurotrophic factor induces phosphorylation of fibroblast growth factor receptor substrate 2.
Brain-derived neurotrophic factor (BDNF) promotes neuronal survival. Gaining an understanding of how BDNF, via the tropomyosin-related kinase B (TRKB) receptor, elicits specific cellular responses is of contemporary interest. Expression of mutant TrkB in fibroblasts, where tyrosine 484 was changed to phenylalanine, abrogated Shc association with TrkB, but only attenuated and did not block BDNF-induced phosphorylation of mitogen-activated protein kinase (MAPK). ... [more]
J. Biol. Chem. Apr. 16, 1999; 274(16);11321-7 [Pubmed: 10196222]
Throughput
- Low Throughput
Curated By
- BioGRID