An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Cullin-3/KCTD10 complex is essential for K27-polyubiquitination of EIF3D in human hepatocellular carcinoma HepG2 cells.

Maekawa M, Hiyoshi H, Nakayama J, Kido K, Sawasaki T, Semba K, Kubota E, Joh T, Higashiyama S

Eukaryotic translation initiation factor 3 subunit D (EIF3D) binds to the 5'-cap of specific mRNAs, initiating their translation into polypeptides. From a pathological standpoint, EIF3D has been observed to be essential for cell growth in various cancer types, and cancer patients with high EIF3D mRNA levels exhibit poor prognosis, indicating involvement of EIF3D in oncogenesis. In this study, we found, ... [more]

Biochem. Biophys. Res. Commun. Dec. 03, 2018; 516(4);1116-1122 [Pubmed: 31280863]

Throughput

High Throughput

Additional Notes

assayed using LC-MS/MS (liquid chromatography/tandem mass spectrometry)
interaction detected in HUVEC cells

Curated By

BioGRID

Interaction Summary

KCTD10

Gene Ontology Biological Process

Gene Ontology Molecular Function

ubiquitin-protein transferase activity [ISS]

Gene Ontology Cellular Component

ATAD3A