BAIT

KCNJ3

GIRK1, KGA, KIR3.1
potassium inwardly-rectifying channel, subfamily J, member 3
GO Process (4)
GO Function (2)
GO Component (2)
Homo sapiens
PREY

KCNJ3

GIRK1, KGA, KIR3.1
potassium inwardly-rectifying channel, subfamily J, member 3
GO Process (4)
GO Function (2)
GO Component (2)
Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Interactions between GABA-B1 receptors and Kir 3 inwardly rectifying potassium channels.

David M, Richer M, Mamarbachi AM, Villeneuve LR, Dupre DJ, Hebert TE

gamma-aminobutyric acid (GABA) is the principal inhibitory neurotransmitter in the mammalian brain. It acts via both ionotropic GABA-A and metabotropic GABA-B receptors. We evaluated the interaction of receptors with members of the inwardly rectifying potassium (Kir 3) channel family, which also play an important role in neuronal transmission and membrane excitability. These channels are functionally regulated by GABA-B receptors. Possible ... [more]

Cell. Signal. Dec. 01, 2006; 18(12);2172-81 [Pubmed: 16809021]

Throughput

  • Low Throughput

Curated By

  • BioGRID