BAIT
OTULIN
FAM105B, GUM
OTU deubiquitinase with linear linkage specificity
GO Process (7)
GO Function (3)
GO Component (1)
Gene Ontology Biological Process
- canonical Wnt signaling pathway [ISS]
- innate immune response [IMP]
- negative regulation of NF-kappaB transcription factor activity [IDA, IMP]
- negative regulation of inflammatory response [IMP]
- nucleotide-binding oligomerization domain containing 2 signaling pathway [IMP]
- protein linear deubiquitination [IDA]
- sprouting angiogenesis [ISS]
Gene Ontology Molecular Function
Homo sapiens
PREY
NEDD8
NEDD-8
neural precursor cell expressed, developmentally down-regulated 8
GO Process (6)
GO Function (2)
GO Component (2)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Regulation of the endosomal SNX27-retromer by OTULIN.
OTULIN (OTU Deubiquitinase With Linear Linkage Specificity) specifically hydrolyzes methionine1 (Met1)-linked ubiquitin chains conjugated by LUBAC (linear ubiquitin chain assembly complex). Here we report on the mass spectrometric identification of the OTULIN interactor SNX27 (sorting nexin 27), an adaptor of the endosomal retromer complex responsible for protein recycling to the cell surface. The C-terminal PDZ-binding motif (PDZbm) in OTULIN associates with ... [more]
Nat Commun Dec. 20, 2018; 10(1);4320 [Pubmed: 31541095]
Throughput
- High Throughput
Additional Notes
- assayed using LFQ LC-MS/MS (label free quantification liquid chromatography/tandem mass spectrometry)
Curated By
- BioGRID