Far Western

An interaction is detected between a protein immobilized on a membrane and a purified protein probe.

Publication

Raver1, a dual compartment protein, is a ligand for PTB/hnRNPI and microfilament attachment proteins.

Huettelmaier S, Illenberger S, Grosheva I, Ruediger M, Singer RH, Jockusch BM

By screening a yeast two-hybrid library with COOH-terminal fragments of vinculin/metavinculin as the bait, we identified a new protein termed raver1. Raver1 is an 80-kD multidomain protein and widely expressed but to varying amounts in different cell lines. In situ and in vitro, raver1 forms complexes with the microfilament-associated proteins vinculin, metavinculin, and alpha-actinin and colocalizes with vinculin/metavinculin and alpha-actinin ... [more]

J. Cell Biol. Nov. 26, 2001; 155(5);775-86 [Pubmed: 11724819]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
RAVER1 VCL
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Low-BioGRID
-

Curated By

  • BioGRID