Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

COP I domains required for coatomer integrity, and novel interactions with ARF and ARF-GAP.

Eugster A, Frigerio G, Dale M, Duden R

We performed a systematic mapping of interaction domains on COP I subunits to gain novel insights into the architecture of coatomer. Using the two-hybrid system, we characterize the domain structure of the alpha-, beta'-, epsilon-COP and beta-, gamma-, delta-, zeta-COP coatomer subcomplexes and identify links between them that contribute to coatomer integrity. Our results demonstrate that the domain organization of ... [more]

EMBO J. Aug. 01, 2000; 19(15);3905-17 [Pubmed: 10921873]

Throughput

  • Low Throughput

Curated By

  • BioGRID