BAIT

BRCC3

BRCC36, C6.1A, CXorf53, RP11-143H17.2
BRCA1/BRCA2-containing complex, subunit 3
UPS Project
Homo sapiens
PREY

SHMT2

GLYA, HEL-S-51e, SHMT
serine hydroxymethyltransferase 2 (mitochondrial)
GO Process (1)
GO Function (2)
GO Component (6)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens

Reconstituted Complex

An interaction is detected between purified proteins in vitro.

Publication

Structural Basis of BRCC36 Function in DNA Repair and Immune Regulation.

Rabl J, Bunker RD, Schenk AD, Cavadini S, Gill ME, Abdulrahman W, Andres-Pons A, Luijsterburg MS, Ibrahim AFM, Branigan E, Aguirre JD, Marceau AH, Guerillon C, Bouwmeester T, Hassiepen U, Peters AHFM, Renatus M, Gelman L, Rubin SM, Mailand N, van Attikum H, Hay RT, Thomae NH

In mammals, ?100 deubiquitinases act on ?20,000 intracellular ubiquitination sites. Deubiquitinases are commonly regarded as constitutively active, with limited regulatory and targeting capacity. The BRCA1-A and BRISC complexes serve in DNA double-strand break repair and immune signaling and contain the lysine-63 linkage-specific BRCC36 subunit that is functionalized by scaffold subunits ABRAXAS and ABRO1, respectively. The molecular basis underlying BRCA1-A and ... [more]

Mol. Cell Dec. 08, 2018; 75(3);483-497.e9 [Pubmed: 31253574]

Throughput

  • Low Throughput

Additional Notes

  • interaction between immobilized BRISC complex (ABRO1, BRCC36, BRE and MERIT40) and SHMT2 assayed using BLI (biolayer interferometry)

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
SHMT2 BRCC3
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High0.9557BioGRID
2250766
SHMT2 BRCC3
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High0.9907BioGRID
3041570
BRCC3 SHMT2
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High2.94BioGRID
423369

Curated By

  • BioGRID