NAPA
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
VAMP8
Gene Ontology Biological Process
- autophagic vacuole fusion [IMP]
- endocytosis [IBA]
- eosinophil degranulation [IMP]
- exocytosis [IBA]
- membrane organization [TAS]
- mucus secretion [IMP]
- negative regulation of secretion by cell [IDA]
- neutrophil degranulation [IMP]
- positive regulation of histamine secretion by mast cell [IMP]
- post-Golgi vesicle-mediated transport [TAS]
- regulation of protein localization to plasma membrane [IDA]
- vesicle fusion [IBA]
- viral entry into host cell [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- SNARE complex [IDA]
- azurophil granule membrane [IDA]
- cytoplasm [IDA]
- cytosol [IDA]
- early endosome [TAS]
- extracellular vesicular exosome [IDA]
- late endosome membrane [IDA]
- lysosomal membrane [IDA]
- membrane [IDA]
- mucin granule [IDA]
- perinuclear region of cytoplasm [IDA]
- plasma membrane [IDA, TAS]
- recycling endosome [IDA]
- secretory granule membrane [IDA, TAS]
- vesicle [IDA]
Reconstituted Complex
An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.
Publication
Endobrevin, a novel synaptobrevin/VAMP-like protein preferentially associated with the early endosome.
Synaptobrevins/vesicle-associated membrane proteins (VAMPs) together with syntaxins and a synaptosome-associated protein of 25 kDa (SNAP-25) are the main components of a protein complex involved in the docking and/or fusion of synaptic vesicles with the presynaptic membrane. We report here the molecular, biochemical, and cell biological characterization of a novel member of the synaptobrevin/VAMP family. The amino acid sequence of endobrevin ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| VAMP8 NAPA | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | - | BioGRID | 3374652 | |
| NAPA VAMP8 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | 1 | BioGRID | 3172019 |
Curated By
- BioGRID