INSR
Gene Ontology Biological Process
- G-protein coupled receptor signaling pathway [IDA]
- activation of MAPK activity [IMP]
- activation of protein kinase B activity [IDA]
- activation of protein kinase activity [IMP]
- cellular response to insulin stimulus [IDA]
- glucose homeostasis [IMP]
- heart morphogenesis [IMP]
- insulin receptor signaling pathway [IDA, TAS]
- peptidyl-tyrosine phosphorylation [IDA]
- positive regulation of DNA replication [IMP]
- positive regulation of MAPK cascade [IMP]
- positive regulation of cell migration [IMP]
- positive regulation of cell proliferation [IC, IDA]
- positive regulation of developmental growth [IMP]
- positive regulation of glucose import [IDA, NAS]
- positive regulation of glycogen biosynthetic process [IDA]
- positive regulation of glycolytic process [IMP]
- positive regulation of mitosis [IMP]
- positive regulation of nitric oxide biosynthetic process [IMP]
- positive regulation of protein kinase B signaling [IMP]
- positive regulation of protein phosphorylation [IDA]
- positive regulation of respiratory burst [IDA]
- protein autophosphorylation [IDA, IMP]
- protein heterotetramerization [IDA]
- regulation of embryonic development [IMP]
- regulation of transcription, DNA-templated [IMP]
- signal transduction by phosphorylation [IDA]
- transformation of host cell by virus [IMP]
Gene Ontology Molecular Function- ATP binding [IDA]
- GTP binding [IDA]
- PTB domain binding [IPI]
- insulin binding [IDA, IPI]
- insulin receptor substrate binding [IPI]
- insulin-activated receptor activity [IDA]
- insulin-like growth factor I binding [IPI]
- insulin-like growth factor II binding [IPI]
- insulin-like growth factor receptor binding [IDA]
- phosphatidylinositol 3-kinase binding [IPI]
- protein binding [IPI]
- protein tyrosine kinase activity [IDA, IMP]
- receptor signaling protein tyrosine kinase activity [IDA]
- ATP binding [IDA]
- GTP binding [IDA]
- PTB domain binding [IPI]
- insulin binding [IDA, IPI]
- insulin receptor substrate binding [IPI]
- insulin-activated receptor activity [IDA]
- insulin-like growth factor I binding [IPI]
- insulin-like growth factor II binding [IPI]
- insulin-like growth factor receptor binding [IDA]
- phosphatidylinositol 3-kinase binding [IPI]
- protein binding [IPI]
- protein tyrosine kinase activity [IDA, IMP]
- receptor signaling protein tyrosine kinase activity [IDA]
Gene Ontology Cellular Component
IRS1
Gene Ontology Biological Process
- Fc-epsilon receptor signaling pathway [TAS]
- JAK-STAT cascade involved in growth hormone signaling pathway [TAS]
- cellular response to insulin stimulus [IMP]
- epidermal growth factor receptor signaling pathway [TAS]
- fibroblast growth factor receptor signaling pathway [TAS]
- glucose homeostasis [TAS]
- innate immune response [TAS]
- insulin receptor signaling pathway [IDA, IMP, IPI, TAS]
- insulin-like growth factor receptor signaling pathway [IPI]
- negative regulation of insulin receptor signaling pathway [ISS]
- negative regulation of insulin secretion [IDA]
- neurotrophin TRK receptor signaling pathway [TAS]
- phosphatidylinositol 3-kinase signaling [IDA]
- phosphatidylinositol-mediated signaling [TAS]
- positive regulation of cell proliferation [NAS]
- positive regulation of fatty acid beta-oxidation [IMP]
- positive regulation of glucose import [IMP]
- positive regulation of glucose import in response to insulin stimulus [IDA]
- positive regulation of glucose metabolic process [IMP]
- positive regulation of glycogen biosynthetic process [IMP, NAS]
- positive regulation of insulin receptor signaling pathway [ISS]
- positive regulation of phosphatidylinositol 3-kinase activity [ISS]
- response to insulin [IDA]
- response to peptide hormone [ISS]
- signal transduction [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Two-hybrid
Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.
Publication
Insulin receptor substrate-2 binds to the insulin receptor through its phosphotyrosine-binding domain and through a newly identified domain comprising amino acids 591-786.
We compared the interaction between the insulin receptor (IR) and the IR substrate (IRS) proteins IRS-1 and IRS-2) using the yeast two-hybrid system. Both IRS proteins interact specifically with the cytoplasmic portion of the IR and the related insulin-like growth factor-I receptor, and these interactions require receptor tyrosine kinase activity. Alignment of IRS-1 and IRS-2 revealed two conserved domains at ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| INSR IRS1 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| IRS1 INSR | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| IRS1 INSR | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| INSR IRS1 | Biochemical Activity Biochemical Activity An interaction is inferred from the biochemical effect of one protein upon another, for example, GTP-GDP exchange activity or phosphorylation of a substrate by a kinase. The bait protein executes the activity on the substrate hit protein. A Modification value is recorded for interactions of this type with the possible values Phosphorylation, Ubiquitination, Sumoylation, Dephosphorylation, Methylation, Prenylation, Acetylation, Deubiquitination, Proteolytic Processing, Glucosylation, Nedd(Rub1)ylation, Deacetylation, No Modification, Demethylation. | Low | - | BioGRID | 824662 | |
| IRS1 INSR | Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro. | Low | - | BioGRID | - | |
| INSR IRS1 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | BioGRID | - |
Curated By
- BioGRID