BAIT

STUB1

CHIP, HSPABP2, NY-CO-7, SCAR16, SDCCAG7, UBOX1, LA16c-313D11.6
STIP1 homology and U-box containing protein 1, E3 ubiquitin protein ligase
GO Process (15)
GO Function (15)
GO Component (10)
Homo sapiens

Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

Specificity for latent C termini links the E3 ubiquitin ligase CHIP to caspases.

Ravalin M, Theofilas P, Basu K, Opoku-Nsiah KA, Assimon VA, Medina-Cleghorn D, Chen YF, Bohn MF, Arkin M, Grinberg LT, Craik CS, Gestwicki JE

Protein-protein interactions between E3 ubiquitin ligases and protein termini help shape the proteome. These interactions are sensitive to proteolysis, which alters the ensemble of cellular N and C termini. Here we describe a mechanism wherein caspase activity reveals latent C termini that are then recognized by the E3 ubiquitin ligase CHIP. Using expanded knowledge of CHIP's binding specificity, we predicted ... [more]

Nat. Chem. Biol. Dec. 01, 2018; 15(8);786-794 [Pubmed: 31320752]

Throughput

  • Low Throughput

Additional Notes

  • assayed using competitive FP (fluorescence polarization) and DSF (differential scanning fluorimetry)

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
STUB1 CASP6
Protein-peptide
Protein-peptide

An interaction is detected between a protein and a peptide derived from an interaction partner. This includes phage display experiments.

Low-BioGRID
2756511

Curated By

  • BioGRID