BAIT

PAFAH1B3

Pafahg, mus[g]
platelet-activating factor acetylhydrolase, isoform 1b, subunit 3
GO Process (2)
GO Function (3)
GO Component (4)
Mus musculus
PREY

PAFAH1B1

LIS-1, Lis1, MMS10-U, Mdsh, Ms10u, Pafaha, RP23-194P5.2
platelet-activating factor acetylhydrolase, isoform 1b, subunit 1
GO Process (35)
GO Function (7)
GO Component (22)
Mus musculus

Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

NudE-L, a novel Lis1-interacting protein, belongs to a family of vertebrate coiled-coil proteins.

Sweeney KJ, Prokscha A, Eichele G

The LIS1-encoded protein (Lis1) plays a role in brain development because a hemizygous deletion or mutation of the human gene causes neuronal migration disorders, such as Miller-Dieker syndrome (MDS) or isolated lissencephaly sequence (ILS). Using a yeast two-hybrid screen, we have isolated a novel protein that interacts with mouse Lis1 (mLis1) which is termed mouse NudE-like protein (mNudE-L) because of ... [more]

Mech. Dev. Mar. 01, 2001; 101(1);21-33 [Pubmed: 11231056]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
PAFAH1B1 PAFAH1B3
Co-fractionation
Co-fractionation

Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex.

High0.8365BioGRID
2671999
PAFAH1B1 PAFAH1B3
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Low-BioGRID
-

Curated By

  • BioGRID