Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Connecting the Dots in the Neuroglobin-Protein Interaction Network of an Unstressed and Ferroptotic Cell Death Neuroblastoma Model.

Van Acker ZP, Van Raemdonck GA, Logie E, Van Acker SI, Baggerman G, Vanden Berghe W, Ponsaerts P, Dewilde S

Neuroglobin is a heme protein of which increased levels provide neuroprotection against amyloid proteinopathy and hemorrhagic damage. These cellular stressors involve the promotion of ferroptosis-an iron-dependent, lipid peroxide-accreting form of cell death. Hence, we questioned whether neuroglobin could oppose ferroptosis initiation. We detected human neuroglobin (hNgb)-EGFP-expressing SH-SY5Y cells to be significantly more resistant to ferroptosis induction, identifying 0.68-fold less cell ... [more]

Cells Dec. 11, 2018; 8(8); [Pubmed: 31405213]

Throughput

  • High Throughput

Additional Notes

  • identified in both SILAC IP (stable isotope labeling by amino acids in cell culture-based quantitative immunoprecipitation) and non-SILAC IP experiments

Curated By

  • BioGRID