Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

SMAP, an Smg GDS-associating protein having arm repeats and phosphorylated by Src tyrosine kinase.

Shimizu K, Kawabe H, Minami S, Honda T, Takaishi K, Shirataki H, Takai Y

Smg GDS is a regulator having two activities on a group of small G proteins including the Rho and Rap1 family members and Ki-Ras; one is to stimulate their GDP/GTP exchange reactions, and the other is to inhibit their interactions with membranes. Structurally, it has 11 Arm repeats, a protein interaction motif, found in the Drosophila Armadillo protein, a homolog ... [more]

J. Biol. Chem. Oct. 25, 1996; 271(43);27013-7 [Pubmed: 8900189]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
RAP1GDS1 KIFAP3	Reconstituted Complex Reconstituted Complex An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.	Shimizu K (1996)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

RAP1GDS1

Gene Ontology Biological Process

Gene Ontology Molecular Function

GTPase activator activity [NAS]

Gene Ontology Cellular Component

KIFAP3