PREY
CAD
carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase
GO Process (11)
GO Function (10)
GO Component (10)
Gene Ontology Biological Process
- 'de novo' pyrimidine nucleobase biosynthetic process [IDA, ISS]
- arginine biosynthetic process [IBA]
- drug metabolic process [ISS]
- glutamine metabolic process [ISS]
- nucleobase-containing small molecule metabolic process [TAS]
- peptidyl-threonine phosphorylation [ISS]
- protein autophosphorylation [ISS]
- pyrimidine nucleobase metabolic process [TAS]
- pyrimidine nucleoside biosynthetic process [TAS]
- small molecule metabolic process [TAS]
- urea cycle [IBA]
Gene Ontology Molecular Function- ATP binding [ISS]
- aspartate binding [ISS]
- aspartate carbamoyltransferase activity [IBA, ISS, TAS]
- carbamoyl-phosphate synthase (ammonia) activity [IBA]
- carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity [IBA, ISS, TAS]
- dihydroorotase activity [IDA, ISS, TAS]
- enzyme binding [IPI]
- identical protein binding [ISS]
- protein kinase activity [ISS]
- zinc ion binding [IDA]
- ATP binding [ISS]
- aspartate binding [ISS]
- aspartate carbamoyltransferase activity [IBA, ISS, TAS]
- carbamoyl-phosphate synthase (ammonia) activity [IBA]
- carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity [IBA, ISS, TAS]
- dihydroorotase activity [IDA, ISS, TAS]
- enzyme binding [IPI]
- identical protein binding [ISS]
- protein kinase activity [ISS]
- zinc ion binding [IDA]
Gene Ontology Cellular Component
Homo sapiens
Proximity Label-MS
An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.
Publication
Identification of phagocytosis regulators using magnetic genome-wide CRISPR screens.
Phagocytosis is required for a broad range of physiological functions, from pathogen defense to tissue homeostasis, but the mechanisms required for phagocytosis of diverse substrates remain incompletely understood. Here, we developed a rapid magnet-based phenotypic screening strategy, and performed eight genome-wide CRISPR screens in human cells to identify genes regulating phagocytosis of distinct substrates. After validating select hits in focused ... [more]
Nat. Genet. Dec. 01, 2017; 50(12);1716-1727 [Pubmed: 30397336]
Throughput
- High Throughput
Additional Notes
- BioID
- SAINT score thresold >/= 8
Curated By
- BioGRID